Identification of two teleost homologs of the Drosophila sex determination factor, transformer-2 in medaka (Oryzias latipes)

Sex determination in fish: Lessons from the sex-determining gene of the teleost medaka, Oryzias latipes.

Although sex determination systems in animals are diverse, sex-determining genes have been identified only in mammals and some invertebrates. Recently, DMY (DM domain gene on the Y chromosome) has been found in the sex-determining region on the Y chromosome of the teleost medaka fish, Oryzias latipes. Functional and expression analyses of DMY show it to be the leading candidate for the male-det...

Cloning and expression of medaka Dachshund

The nuclear factor dachshund (dac) is a regulator of retinal cell fate determination in Drosophila. We have cloned a Dachshund homologue of a lower vertebrate, the teleost medaka (Oryzias latipes). Sequence comparison reveals high similarity of medaka Dachshund (OlDach) to the known homologues in higher vertebrates and Drosophila. OlDach is first expressed at early somitogenesis stages in the o...

Cloning and developmental expression patterns of Dlx2, Lhx7 and Lhx9 in the medaka fish (Oryzias latipes)

We have isolated three homeodomain and LIM-homeodomain developmental transcription factors from the medaka fish (Oryzias latipes): OlDlx2, OlLhx7, and OlLhx9, and we have studied their expression patterns in the developing and adult brain. This analysis showed that OlDlx2 and OlLhx7 (together with OlNkx2.1b) delineate the subpallial divisions of the medaka telencephalon, and that OlLhx9 exhibit...

Developmentally regulated and non-sex-specific expression of autosomal dmrt genes in embryos of the Medaka fish (Oryzias latipes)

The dmrtgene family of vertebrates comprises several transcription factors that share a highly conserved DNA-binding domain, the DM domain. Like some of their invertebrate counterparts, e.g. Drosophila doublesex (dsx) and the Caenorhabditis elegans Mab3, several are implicated in sex determination and differentiation. Thus far, dmrt genes represent the only factors involved in sexual developmen...

Genetic Approaches to Understanding the Basic Mechanisms of Endocrine Disruptor Actions on Sex Determination/Differentiation in the Medaka, Oryzias latipes